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. 2014 Dec 31;9(12):e116229. doi: 10.1371/journal.pone.0116229

Figure 1. Experimental designs.

Figure 1

(A) CP was induced by administration of 6 i.p. injection of cerulein (50 ug/kg) at 1-h interval, 2 days per week, for a total of 6 weeks. Control female mice received NS in a similar manner. G-CSF (200 ug/kg/day) or NS was then i.p injected once a day for 5 consecutive days. Groups of mice were sacrificed at 7 (W7 groups) and 9 (W9 groups) weeks after the beginning of cerulein treatment, and the pancreases were removed and processed for further analysis. (B) Sex mismatched BM transplantation was performed using 8-week-old WT C57BL/6 female mice as recipients and 8-week-old male GFP mice as donors after 5 Gy whole body gamma irradiation to ablate their BM. Six weeks after transplantation, Mice (W0 group) were sacrificed to determine the success of re-established haematopoietic system by transplanted male GFP BM. The mice were then treated in the same experimental design mentioned in A.