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. 2014 Dec 31;9(12):e116137. doi: 10.1371/journal.pone.0116137

Figure 3. Trypanosoma cruzi genotyping from naturally infected dogs and triatomines from the Amazon Biome.

Figure 3

(A) PCR product size polymorphisms of the non-transcribed intergenic region of the SL-RNA mini-exon (mini-exon assay) of T. cruzi DNA from dog sera from Monte Alegre in the state of Pará, samples: 1- dog LBT 1818, 2- dog LBT 1819, 3- dog LBT 1820, 4- dog LBT 1821, and 5- dog LBT 1822 (B) Mini-exon assay of Rhodnius pictipes isolates from Abaetetuba (LBT 1458) and Belém (LBT 1814) in the state of Pará, Samples: 1- LBT 1458 and 2- LBT 1814 (C) T. cruzi genotyping profiles for PCR-RFLP with the 1f8 gene/Alw21I restriction enzyme of LBT 1458 clones 5 and 7, Samples: 1- LBT 1458 clone 5 and 2- LBT 1458 clone 7 (D) T. cruzi genotyping profiles for PCR-RFLP with histone 3/AluI restriction enzyme for the LBT 1814 isolate: Sample 1- LBT 1814 isolate. The T. cruzi DTUs, T. rangeli (H-14) and negative controls are indicated in the figure. DTU reference strains: I - Sylvio X/10 cl 1; II – Esmeraldo cl3; III – M5631 cl5; IV–92122102R; V – SC43 cl1; and VI – CL Brener. Agarose gel 3%, stained with ethidium bromide.