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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Kidney Int. 2014 Jul 30;87(1):151–161. doi: 10.1038/ki.2014.268

Figure 2. RNase 6 and RNase 7 rapidly kill uropathogenic bacteria at micromolar concentrations.

Figure 2

Uropathogenic E. faecalis (A), S. saprophyticus (B), and E. coli strains CFT073 and UTI89 (C,D) were stained using a 1:1 mixture of SYTO®9, which labels live bacteria, and propidium iodide, which labels killed bacteria. Bacteria were incubated with fixed concentrations of recombinant murine RNase 6, human RNase 6, or RNase 7. (E/F) E. coli strain UTI89 was stained as outlined above and incubated with increasing concentrations of recombinant human or mouse RNase 6. Untreated bacteria served as the control. Bacterial viability over time was analyzed integrating fluorescent changes in SYTO®9 dye and propidium iodide dye. Values are the average of three replicates.