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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Kidney Int. 2014 Jul 30;87(1):151–161. doi: 10.1038/ki.2014.268

Figure 3. RNase 6 and RNase 7 mRNA and peptide expression in the human urinary tract.

Figure 3

(A) Quantification of human RNASE6 and RNASE7 mRNA in non-infected tissues and pyelonephritis kidney samples by qRT-PCR. (B) Western immunoblot analysis of human RNase 6 (R6) and RNase 7 (R7) protein in bladder (B), non-infected kidney (HK) and pyelonephritis (P) lysates. GAPDH is included as a loading control. (C) Immunoblot analysis of human RNase 6 (R6) and RNase 7 (R7) in sterile (St) or UPEC infected (I) human urine (left). Infected human urine was subjected to centrifugation and immunoblot analysis was repeated using the cellular pellet (P) and supernatant (S) fractions (right).