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. 2014 Nov;47(11):649–654. doi: 10.5483/BMBRep.2014.47.11.185

Fig. 3. L-alanine or L-serine strongly blocks LPS-induced signaling in mouse neutrophils. (A) Neutrophils were stimulated with vehicle (PBS), L-alanine (10 mM, 100 mM), or L-serine (10 mM, 100 mM) for 30 min, and were stimulated with PBS or LPS (1 μg/ml) for 24 h. TNF-α, CCL-2, and IL-10 levels were measured by ELISA. Data are expressed as the mean±SEM (n=3). *P<0.05, **P<0.01, ***P<0.001, compared with the vehicle control (A). Neutrophils were stimulated with vehicle or L-serine (100 mM, for B) or L-alanine (100 mM, for C) for 30 min, and were stimulated with PBS or LPS (1 μg/ml) for 0, 30, 60, 90, or 120 min. The levels of I-κB, phosphorylated Akt or phosphorylated STAT3 were measured by Western blot analysis. The data represents three independent experiments (B and C).

Fig. 3.