Fig. 1.
SDS-PAGE analysis of purification of recombinant G. lamblia GlcN6P deaminase overexpressed in E. coli BL21(DE3) pLysS cells. a Wild-type GlmNagB; lane 1—total lysate; lane 2—streptomycin sulfate precipitation; lane 3—ammonium sulfate precipitation; lane 4—PEG precipitation (dissolved sediment); lane 5—ion exchange chromatography on Resource Q; lane M—molecular mass markers. b GlmNagB-HisC; lane 1—total lysate; lane 2—wash of the Ni2+-IDA column with 0–50 mM imidazole; lane 3—200 mM imidazole eluate; lane M—molecular mass markers