Table 1.

Summary of the purification of GlmNagB overexpressed in Escherichia coli BL21(DE3) pLysS

Step	Volume [ml]	Total protein [mg]	Specific activity [U/mg]	Total activity [U]	Purification factor	Yield [%]
Crude extract	20	360	8.6	3,096	1	100
Streptomycin sulfate	22	210	13.5	2,835	1.6	91.6
Ammonium sulfate	11	65	24	1,555	2.8	50.2
PEG	9	29.2	32	936	3.7	30.2
Resource Q	6	5.2	62	322	7.2	10.4

The anabolic activity (synthesis of GlcN6P) was determined