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The final purification results of wild-type GlmNagB and its muteins with His6-tagged at C- or N-terminal
| Protein | Total protein [mg/l] | Specific activity [U/mg] |
|---|---|---|
| GlmNagB | 5.2 | 62.0 |
| GlmNagB-HisC | 3.2 | 0.5 |
| GlmNagB-HisN | 14.5 | 73.0 |
Anabolic activity (synthesis of GlcN6P) was determined
