Table 4.

Summary of results of SEC analysis of the wild-type GlmNagB and its oligoHis-tagged muteins

Fraction	Native MW (SEC) [kDa]	MW of the denatured protein (from SDS-PAGE) [kDa]	Specific activity [U/mg]	Putative quaternary structure
GlmNagB
1	131.4 ± 25.0	30.7 ± 2.0	48	Homotetramer
2	58.0 ± 11.0	30.7 ± 2.0	55	Homodimer
3	27.7 ± 5.0	30.7 ± 2.0	51	Monomer
GlmNagB-HisN
1	121.0 ± 24.0	33.0 ± 2.0	97	Homotetramer
2	69.4 ± 14.0	32.6 ± 2.0	89	Homodimer
3	31.1 ± 6.0	32.6 ± 2.0	80	Monomer
GlmNagB-HisC
1	138.7 ± 27.0	32.0 ± 2.0	0.48	Homotetramer
2	59.4 ± 11.0	32.0 ± 2.0	0.50	Homodimer
3	28.4 ± 5.0	32.0 ± 2.0	0.52	Monomer