Table 3.

Basal [³⁵S]GTPγS binding to membranes of mouse brain following various morphine treatments

[35S]GTPγS binding
Naive	Morphine acute	Morphine moderate	Morphine severe
3,618 ± 356	3,057 ± 157	2,857 ± 342	3,361 ± 543

Values are expressed as counts per minute bound per tube and are shown as means ± SEM from 9–11 separate experiments in each case, performed in triplicate or quadruplicate. The values were not significantly different, one-way ANOVA with Bonferroni post-test. The same amount of membrane protein (10 μg/tube) was used in each case