Fig. 3.

a AF-16 was deposited intranasally (0.3 μmol/kg in 25 μl) under Isoflurane anaesthesia, after which the concentration (ng/ml) of the peptide was determined in plasma (filled bars) and in CSF (unfilled bars) after a 15-min- or a 30-min-long period. Higher levels of AF-16 are found in plasma than in CSF at both times. Mean ± SEM. b Autoradiography showing distribution of ¹⁴C-AF-16 in the rat brain 30 min after intranasal administration of the peptide. X-ray film showing signal in the lateral (LV) and third ventricles (3V); hc hippocampus