Graphical representation of the potential Ser and Thr residues for O-β
-GlcNAc as well as yin yang sites in human IRS-1. (a) Predicted potential sites for O-β
-GlcNAc modification of Ser and Thr. Green vertical line shows O-β-GlcNAc modification potential of Ser/Thr residues, while the light blue wavy line indicates the threshold for modification potential. YinOYang 1.2 web based program generated this graph. A total number of 57 potential Ser/Thr residues showed O-β-GlcNAc modification potential including Ser-7, 268, 270, 273, 295, 303, 312, 341, 348, 388, 391, 393, 394, 395, 412, 413, 417, 440, 541, 543, 624, 639, 641, 680, 681, 682, 683, 684, 741, 807, 808, 809, 917, 985, 1000, 1005, 1011, 1025, 1035, 1036, 1037, 1101, 1105, 1213, and 1223 and Thr-305, 311, 351, 387, 539, 743, 803, 847, 851, 1004, 1030, and 1045. (b) Predicted yin yang residues for human IRS-1. A total of 37 Ser/Thr residues showed potential for possible yin yang sites for phosphorylation and O-glycosylation interplay.