Table 1.
Drugs Analyzed for Drug-Drug Suppression
| Drug | Abbreviation | Max Dose (μg/ml) | Experimental Tests and Suppressive Interactions |
|---|---|---|---|
| 1,10-phenanthroline | 110 | 5 | AmB, Ben, Bro, Sta |
| 2-deoxy-D-glucose | 2DG | 250 | Sta |
| 5-fluorouracil | 5FU | 28 | Ben, Lat, Pen, Sta, Tac, Ter |
| 6-azauracil | 6Az | 1,000 | AmB, Ben, Bro, Sta |
| Amphotericin B | AmB | 0.49 | 110, 6Az, Ben, Cer, Cet, Dox, Flu, Mic, Naz, Nys, Pen, Phl, Sta, Tac, Ter |
| Anisomycin | Ani | 3.5 | Ben, Lat, Pen, Sta, Tac, Ter |
| Aureobasidin A | AbA | 280 | Lit, Wor |
| Benomyl | Ben | 28 | 110, 5FU, 6Az, AmB, Ani, Bro, C3P, Cal, Cer, Cet, Chl, Cis, Cyc, Dyc, Dox, Fen, Flu, Hal, Lat, Met, Mic, MMS, Myr, Naz, Nys, Pen, Phl, Qmy, Rad, Rap, Sta, Tac, Ter, Tun |
| Bromopyruvate | Bro | 490–1,000a | 110, 6Az, Ben, Cal, Cer, Cet, Dox, Dyc, Fen, Flu, Hal, Lat, Mic, Naz, Nys, Pen, Phl, Rap, Sta, Tac, Ter, Tun |
| Calyculin A | Cal | 2.1 | Ben, Bro, Dyc, Fen, Hal, Lat, Pen, Rap, Sta, Tac, Ter, Tun |
| Cantharidin | Can | 140 | Lat, Pen, Sta, Tac, Ter |
| Carbonyl cyanide 3-chlorophenylhydrazone | C3P | 21 | Ben, Lat, Pen, Sta, Tac, Ter |
| Cerulenin | Cer | 0.335 | AmB, Ben, Bro, Sta |
| Cetylpryidinium | Cet | 0.8 | AmB, Ben, Bro, Sta |
| Chlorzoxazone | Chl | 350 | Ben, Lat, Pen, Sta, Tac, Ter |
| Cisplatin | Cis | 80 | Ben, Lat, Sta, Tac, Ter |
| Clozapine | Clo | 105 | Rad |
| Cycloheximide | Cyc | 0.91 | Ben, Lat, Pen, Rad, Sta, Tac, Ter |
| Doxorubicin | Dox | 11.6 | AmB, Ben, Bro, Sta |
| Dyclonine | Dyc | 49 | Ben, Bro, Cal, Fen, Hal, Lat, Pen, Rap, Sta, Tac, Ter, Tun |
| Fenpropimorph | Fen | 1.54 | Ben, Bro, Cal, Dyc, Hal, Lat, Pen, Rap, Sta, Tac, Ter, Tun |
| Fluconazole | Flu | 10 | AmB, Ben, Bro, Sta |
| Haloperidol | Hal | 56 | Ben, Bro, Cal, Dyc, Fen, Lat, Pen, Rap, Sta, Tac, Ter, Tun |
| Hygromycin | Hyg | 7 | Lat, Myr, Rad, Rap, Sta |
| Iodoacetamide | Ide | 3 | Sta |
| Iodoacetate | Ite | 500 | Sta |
| Latrunculin B | Lat | 14 | 5FU, Ani, Ben, Bro, C3P, Cal, Can, Chl, Cis, Cyc, Dyc, Fen, Hal, Hyg, Met, MMS, Myr, Pen, Qmy, Rad, Rap, Sta, Tac, Tam, Ter, Tun |
| Lithium | Lit | 4,500 | AbA, Rad, Rap, Sta, Tac |
| Methotrexate | Met | 1,000 | Ben, Lat, Pen, Sta, Tac, Ter |
| Methyl methanesulfonate | MMS | 175 | Ben, Lat, Pen, Sta, Tac, Ter |
| Miconazole | Mic | 0.12 | AmB, Ben, Bro, Sta |
| Myriocin | Myr | 0.350 | Ben, Hyg, Lat, Pen, Qnn, Rad, Sta, Tac, Ter |
| Nystatin | Nys | 2 | AmB, Ben, Bro, Sta |
| Pentachlorophenol | PcP | 28 | Sta |
| Pentamidine | Pen | 70 | 5FU, AmB, Ani, Ben, Bro, C3P, Cal, Can, Chl, Cyc, Dyc, Fen, Hal, Lat, Met, MMS, Myr, Qmy, Rad, Rap, Sta, Tac, Ter, Tun |
| Phleomycin | Phl | 0.00083 | AmB, Ben, Bro, Sta |
| Quinine | Qnn | 1,000 | Myr, Rad |
| Quinomycin | Qmy | 30 | Ben, Lat, Pen, Sta, Tac, Ter |
| Radicicol | Rad | 56 | Ben, Clo, Cyc, Hyg, Lat, Lit, Myr, Pen, Qnn, Rap, Sta, Tac, Ter |
| Rapamycin | Rap | 0.0049 | Ben, Bro, Cal, Dyc, Fen, Hal, Hyg, Lat, Lit, Pen, Rad, Sta, Tac, Ter, Tun |
| Sodium azide | Naz | 25 | AmB, Ben, Bro, Sta |
| Staurosporine | Sta | 1.26 | 110, 2DG, 5FU, 6Az, AmB, Ani, Ben, Bro, C3P, Cal, Can, Cer, Cet, Chl, Cis, Cyc, Dox, Dyc, Fen, Flu, Hal, Hyg, Ide, Ite, Lat, Lit, Met, Mic, MMS, Myr, Naz, Nys, Pcp, Pen, Phl, Qmy, Rad, Rap, Tac, Tam, Ter, Tun, Wor |
| Suppressor of FK506 | Sfk | 3 | Tac |
| Tacrolimus | Tac | 110 | 5FU, AmB, Ani, Ben, Bro, C3P, Cal, Can, Chl, Cis, Cyc, Dyc, Fen, Hal, Lat, Lit, Met, MMS, Myr, Pen, Qmy, Rad, Rap, Sfk, Sta, Tam, Ter, Tun |
| Tamoxifen | Tam | 2.8 | Lat, Sta, Tac, Ter |
| Terbinafine | Ter | 10.5 | 5FU, AmB, Ani, Ben, Bro, C3P, Cal, Can, Chl, Cis, Cyc, Dyc, Fen, Hal, Lat, Met, MMS, Myr, Pen, Qmy, Rad, Rap, Sta, Tac, Tam, Tun |
| Tunicamycin | Tun | 0.35 | Ben, Bro, Cal, Dyc, Fen, Hal, Lat, Pen, Rap, Sta, Tac, Ter |
| Wortmannin | Wor | 280 | AbA, Sta |
Also given are the three-letter abbreviations used in the figures, the maximum dose used, and a list of all the drug interaction experiments made for each drug. The last column indicates the suppressed drug (underlined) and the suppressing drug (shown in bold). Each drug was tested in eight different concentrations, where the lowest concentration is zero, the highest concentration is close to MIC, and increments are evenly spaced between these extremes. Thus, the lowest concentration for each drug tested is 1/7 of the MIC value, and the next is at 2/7 of MIC, etc.
a
Bro MIC was reported as 490 μg/ml in a previous study. In this study, we found Bro MIC as 1,000 μg/ml and used this MIC value in experiments. For all other drugs, the MIC variation was less than 2-fold.