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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Cell Physiol. 2009 Jan;218(1):84–93. doi: 10.1002/jcp.21571

Figure 3.

Figure 3

Ligand-induced Notch signaling effectively blocks myogenesis in cells expressing reduced levels of Hey1. Individual tissue culture wells were treated with Fc-Dll4 or Fc-control as indicated in the Methods. C2C12 cells transfected with either non-silencing (NS) control siRNA oligonucleotides or Hey1-directed siRNAs were propagated on the coated plates and then shifted to DM for 24 hours. Expression of Hey1 (left panel) and Myogenin (right panel) RNA was assessed by quantitative RT-PCR. Q-PCR values are presented as the average +/− standard deviation of three replicate samples. p values were computed by a standard unpaired t-test.