Fig. 1.

Effects of αvβ3 integrin expression on tumor cell growth in vitro. The growth of tumor cells expressing (M21 melanoma and ECV carcinoma) or lacking (M21L melanoma and ECVL carcinoma) αvβ3 integrin was examined in vitro over a 3-day time course. a Quantification of M21 cell variant growth. b Flow cytometry analysis of the relative expression of αvβ3 integrin expressed as the mean fluorescence intensity (MFI) of ECV carcinoma cell variants. ECV-Cont, MFI of parental ECV cells stained with control secondary antibody only. ECV-αvβ3, MFI of parental ECV cells stained with Mab LM609. ECVL-Cont, MFI of ECVL cells derived from four rounds of αvβ3 negative selection and stained with control secondary antibody only. ECVL-αvβ3, MFI of ECVL cells derived from four rounds of αvβ3-negative selection and stained with Mab LM609. c Quantification of ECV cell variant growth. Data bars represent the mean optical density ±SE from triplicate wells. Experiments were completed three times with similar results