Skip to main content
. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Angiogenesis. 2014 Sep 24;18(1):31–46. doi: 10.1007/s10456-014-9445-2

Fig. 3.

Fig. 3

Differential expression of IGFBP-4 in M21 melanoma cell variants. M21 tumor cell variants that express (M21) or lack (M21L) expression of αvβ3 were harvested from sub-confluent cultures. a The relative level of IGFBP-4 mRNA was analyzed by real-time RT-PCR. Data bars represent mean fold change ± SE from four independent experiments. Relative levels of IGFBP-4 in M21 cells were set to 1.0 for each experiment. b Analysis of IGFBP-4 protein in conditioned medium (CM) and whole cell lysates (LY) from M21 cell variants by Western blot. * Indicates P values <0.05