Fig. 5.

Reduced angiogenesis associated with M21 tumors over-expressing IGFBP-4. M21 cells were transfected with control construct (M21Con) or IGFBP-4 expression construct (M21BP4). a Western blot analysis of conditioned medium (CM) and whole cell lysates (LY) for IGFBP-4 expression. b M21 cells (0.5 × 10⁶) overexpressing IGFBP-4 (M21BP4) or control-transfected cells (M21Con) were injected into mice, and tumors were allowed to form for 7 days. Tumor-associated angiogenesis was assessed by quantifying surface vessels infiltrating the tumors from the surrounding tissue. Top panel Examples of vessels infiltrating tumors from each condition. Middle panel Example of tissues from each tumor variant stained by H&E. Bottom panel Quantification of vessel infiltration of M21 melanomas from each condition. Data bars indicate mean number of surface vessels infiltrating the tumors ±SE from 3–4 tumors from each condition. c Tumor cell variants (5 × 10⁶) were injected and tumors allowed to form for 7 days. Top panel Quantification of tumor size from each cell variant. Data bars represent mean tumor volumes ± SE from 7 to 8 tumors per condition. Bottom panel Example of tissues from each tumor variant stained by H&E. d Quantification of vessel infiltration of M21 melanomas from each condition. Data bars indicate mean number of surface vessels infiltrating the tumors ±SE from 7 to 8 tumors from each condition. *Indicates P values <0.05