Fig. 6.

Regulation of IGFBP-4 expression in M21 cells involves altered p38 MAPK signaling. M21 melanoma cells expressing (M21) or lacking (M21L) αvβ3 were examined for the expression of total and activated p38 MAPK and Erk. a Western blots of the levels of total and phosphorylated p38 MAPK and Erk in M21 cell variant lysates. b Western blots of the levels of p38 MAPK isoforms in M21 cell variant lysates. c, d M21 cells were incubated with p38 inhibitor (P38IN) or control DMSO. The levels of IGFBP-4 were assessed by Western blot (c) or real-time RT-PCR (d). Experiments were completed 2–3 times with similar results. *Indicates P values <0.05