Figure 5. Biophysical properties of recombinant human RyR2-H29D mutant.

A: Bar graph summarizing single channel Po under 150, 350 and 700 nmol/L cytosolic [Ca²⁺] in RyR2-WT (n = 5), RyR2-H29D (n = 11) channels. B: Bar graph summarizing the frequency of openings (events/min) under 150, 350 and 700 nmol/L cytosolic [Ca²⁺] in RyR2-WT (n = 5), RyR2-H29D (n = 11) channels. C: Normalized single channel open probability of mutant and WT channels were measured at Ca²⁺ concentrations from 150 nmol/L to 10 µmol/L. Each data point represents the open probability calculated as an average from 3 to 10 independent experiments and divided by the maximal open probability obtained from 150 nmol/L to 10 µmol/L. Data presented as mean ± S.E.M; *p < 0.05; **p < 0.01.