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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Cell Physiol. 2015 Feb;230(2):366–377. doi: 10.1002/jcp.24717

Figure 4. Silencing of MT1-MMP expression correlates with ERK1/2 activation by FGF-2 in Res186 pilocytic astrocytoma cells.

Figure 4

Western blotting analysis of MT1-MMP expression and ERK1/2 activation (p-ERK1/2). A. Res186 cells transiently transfected with the MT1-MMP siRNAs pool or control scrambled siRNAs were grown for 24 h in medium containing 0.5% FBS and incubated with FGF-2 (30 ng/ml) for 15 min. Tubulin (TUB) and ERK2 are shown as loading control. B. Mean ± S.E. of densitometric readings normalized to the corresponding loading control; *, p ≤ 0.05; MT1-MMP siRNA transfectants + FGF-2 vs. control siRNA transfectants + FGF-2. This experiment was repeated twice with similar results.