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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Cell Physiol. 2015 Feb;230(2):366–377. doi: 10.1002/jcp.24717

Figure 5. FGF-2 binding to cells inversely correlates with MT1-MMP expression.

Figure 5

A. MT1-MMP Tet-Off MCF-7 cells grown for 24 h in the presence or absence of DOX (1 μg/ml) in medium containing 0.5% FBS were incubated with FGF-2 (30 ng/ml) for the indicated time. ERK1/2 phosphorylation, MT1-MMP expression and cell-associated or soluble FGF-2 in the conditioned medium were characterized by Western blotting. Tubulin (TUB) is shown as a loading control. B. Densitometric analysis of ERK1/2 activation and cell extract-associated FGF-2. The histograms show mean ± S.E. of densitometric readings normalized to the corresponding loading control; *, p ≤ 0.05; - MT1-MMP vs. the corresponding + MT1-MMP sample. This experiment was repeated three times with similar results.