Skip to main content
. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: J Mol Biol. 2014 Jul 1;427(6 0 0):1211–1223. doi: 10.1016/j.jmb.2014.06.016

Figure 11.

Figure 11

A. Record of trans addition of 0.47 µM LFN(1–16)-YFP to a membrane containing (PA63F427A)7 channels. After the current (shown as a black line) produced by (PA63F427A)7 channels reached a steady state at −20 mV, LFN(1–16)-YFP was added at the green arrow (voltage held at −20 mV), reducing the current by a factor of ~11. The zero baseline of current is shown as a dashed red line. B. Single-channel records of the trans effect of LFN(1–16)-YFP on the conductance of membranes containing one (PA63F427A)7 channel (top) and one WT (PA63)7 channel (bottom) at −20 mV. Note the intermediate substates in both records. (The intermediates are smaller in the WT channel as its conductance is roughly half that of the mutant channel.) Subsequent unblocking at +20 mV (not shown) confirmed that the channel was still present. The two dashed red lines in each record represent, respectively, the zero baseline of conductance and the open state conductance of one channel. Record is 35 seconds long and filtered at 10 Hz.