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. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: J Mol Biol. 2014 Jul 1;427(6 0 0):1211–1223. doi: 10.1016/j.jmb.2014.06.016

Figure 5.

Figure 5

Left. Cis effect of saturating concentrations (~0.47 µM) of truncated His-LFN-YFP constructs on conductance of (PA63F427A)7 channels at +20 and +50 mV. All experiments were performed in triplicate; error bars represent standard error (bars that are not visible are small enough that they are obscured by the marker). Right. List of truncated His-LFN-YFP constructs and the number of amino acids/length of each (not including YFP, which is presumed not to unwind when positive voltages are applied). All of the constructs contain a 20-residue His6-tag, as well as a Ser-Gly-Ser linker before YFP, except for His-YFP (the first construct on the list), which is just the His6-tag attached directly to YFP.