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. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: J Mol Biol. 2014 Jul 1;427(6 0 0):1211–1223. doi: 10.1016/j.jmb.2014.06.016

Figure 6.

Figure 6

Left. Cis effect of saturating concentrations (~0.47 ~M) of truncated LFN-YFP constructs on the conductance of (PA63F427A)7 channels at +20 mV and +50 mV. All experiments were performed in triplicate; error bars represent standard error (bars that are not visible are small enough that they are obscured by the marker). Right. List of truncated LFN-YFP constructs and the number of amino acids/length of each (not including YFP, which is presumed not to unwind when positive voltages are applied). All of the constructs contain four residual residues from the His6-tag (which was cleaved by thrombin), followed by the specified LFN residues as well as a Ser-Gly-Ser linker before YFP.