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. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: J Mol Biol. 2014 Jul 1;427(6 0 0):1211–1223. doi: 10.1016/j.jmb.2014.06.016

Figure 7.

Figure 7

Single-channel records of cis effect of LFN-YFP on conductance of (PA63F427A)7 (top) and WT (PA63)7 (bottom) channels at +20 mV and +50 mV. For each record, the upper dashed blue line represents the open state and the lower dashed red line represents the completely blocked (or closed) state. (The open-state conductance of the mutant channel is roughly double that of the WT channel at both +20 mV and +50 mV.) Note that the conductance of the WT (PA63)7 channel is completely blocked by LFN-YFP, whereas there is a small residual conductance at both +20 mV and +50 mV in the (PA63F427A)7 channel. Records are 35 seconds long and filtered at 15 Hz.