Fig. 4.
Cranial skeletal morphology. Alizarin red and Alcian blue skeleton preparations of E17.5 wild-type control (A-C, G) and homozygous mutant Crklsno/sno (D-F, H) embryos. Lateral view of control (A) and mutant (D) illustrating the diminutive mandibular skeleton (arrowhead). Ventral view of control (B) and mutant (E) skull, and control (C) and mutant (F) isolated mandible. Ventral view of anterior skull base of control (G) and mutant (H) illustrating the impact of Crkl mutation on the palate and maxillary arch skeleton. The mutant skeleton preparation shown here (D-F, H) is from the same embryo shown in whole mount in figure 2 C-E. BS = Basisphenoid; TB = tympanic bulla; BO = basioccipital; PMx = pre-maxilla; Mx = maxilla; Z = zygomatic arch; P = palatine; PP = pre-palatine.