Fig. 4.
Mineralization due to P2X7R activation is amplified in CAV1 KD cells. A: immunoblot showing extent of CAV1 suppression in stable MC3T3-E1 CAV1 KD cells generated using shRNA. B: mineralization nodules observed after von Kossa staining on MC3T3-E1 cells after 21 days. Addition of 150 μM BzATP clearly increased mineralization in CAV1 KD cells, as seen by the nodules. C: extent of mineralization shows a significant increase CAV1 KD cells with BzATP treatment. *P < 0.001. D: Alizarin red staining shows a strong increase in mineralization in CAV1 KD cells treated with BzATP compared with untreated (vehicle) cells. E: extent of mineralization was quantified using ImageJ. aP < 0.05 vs. control.