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. 2014 Nov 7;308(1):H39–H48. doi: 10.1152/ajpheart.00330.2014

Fig. 5.

Fig. 5.

ANT1 knockdown increases mitochondrial reactive oxygen species (mtROS) in H9c2 cells. Confocal microscopy analysis of mtROS in control and ANT1 siRNA-transfected cells treated with either vehicle or TNFα (10 ng/ml) for 24 h is shown. Cells were loaded with MitoSox Red. Representative images from cells transfected with control siRNA treated with vehicle (A), control siRNA treated with TNFα (B), ANT1 siRNA treated with vehicle (C), and ANT1 siRNA treated with TNFα (D) are shown. Bar = 50 μm. E: quantified results for mtROS from 3 independent experiments in control siRNA- or ANT1 siRNA-transfected cells treated with either vehicle or TNFα. *P < 0.01 vs. control siRNA treated with vehicle; **P < 0.01 vs. control siRNA treated with TNFα; #P < 0.05 vs. vehicle.