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. 2014 Nov 5;308(1):F69–F75. doi: 10.1152/ajprenal.00484.2014

Fig. 2.

Fig. 2.

Blockade of HMGB1 release attenuated UUO-induced kidney structural damage. Mice received either saline or glycyrrhizic acid monosodium (GA) treatment (GAT) as described in experimental procedures and then underwent sham operation (sham) or UUO for 7 days. A: HMGB1 expression was detected by immunostaining. B: HMGB1 protein expression was detected by Western blot (top) and quantified by normalization to α-tubulin expression (bottom). C: macrophage infiltration in the interstitium was detected by immunostaining of F4/80. D: quantitative analysis of the expression of T cell marker CD3 and macrophage marker F4/80 by quantitative RT-PCR. E and F: kidney structure (E) and injury score (F) were assessed by hematoxylin and eosin staining. G: serum HMGB1 concentration was measured by ELISA. H: urine HMGB1 concentration was measured by ELISA. n = 5. *P < 0.01 compared with the sham group; #P < 0.01 compared with the UUO group.