FIGURE 5.

H69 cells stably expressing miR-506 show elevated miR-506 expression (A) and exhibit markedly decreased InsP₃R3 mRNA (B) and protein (C) levels. H69 normal cholangiocyte cells stably expressing a negative control miR or miR-506 were generated as described under “Experimental Procedures.” A, mature miR-506 levels in H69-miR-506 cells and control-miR cells were analyzed by TaqMan qPCR and normalized to U6 snoRNA. B, InsP₃R3 mRNA levels were analyzed in both cell types by qPCR using TaqMan assay and expressed relative to negative controls after normalization to β-actin. C, InsP₃R3 protein levels were examined from protein extracts of negative control-miR and miR-506 H69 at two different passages (two N and P lanes). Gapdh protein levels were used as loading controls. The two lanes for the N (control-miR) and P (H69 miR-506 cells) correspond to passage numbers 9, 15 and 6, 10, respectively. Rel.Exp, relative expression compared with control-miR.