TABLE 1.

Oligonucleotides used in InsP₃R3–3′-UTR constructs and in the mutagenesis of miR-506 binding sites

Oligo name	Sequence 5′ → 3′
InsP3R3–3′-UTR Fa	ATGCTCATCACTGGAGACTGCG
InsP3R3–3′-UTR	AGGGGCTCTTGTTCCTGTCTTCTG
3′-UTR PWF	CGCGTCACTGGGACTCAGTTTACCTTAAT
	GCCTTAGCAGAAGATAAATCCTAAGA
3′-UTR PWR	AGCTTTTCTAGGAGGATTTATCTTCTGC
	TAAGGCATTAAGGTAAACTGAGTCCCAGTG
3′-UTR PMF	CGCGTCACTGGGACTCAGTTTACCTTAATG
	AAGTAGCAGAAGATAAATCCTAAGA
3′-UTR PMR	AGCTTTCTTAGGATTTATCTTCTGCTA[i]CTTC
	ATTAAGGTAAACTGAGTCCCAGTG
3′-UTR DWF	CGCGTCAAATCCTTTGGCCTGAGAACTAAT
	ATGTTAATTGCCTTAAATAAATTAATAGAAATCTAGTCCGTTAAAAA
3′-UTR DWR	AGCTTTTTTTAACGGACTAGATTTCTATTAA
	TTTATTTAAGGCAATTAACATATTAGTTCTCAGGCCAAAGGATTTG
3′-UTR DMF	CGCGTCAAATCCTTTGGCCTGAGAACTAAT
	ATGTTAATTG[i]AAGTAAATAAATTAATAGAAATCTAGTCCGTTAAAAA
3′-UTR DMR	AGCTTTTTTTTAACGGACTAGATTTCTATTA
	ATTTATTTA[i]CTTCAATTAACATATTAGTTCTCAGGCCAAAGGATTG

^a The following abbreviations are used: F, forward; R, reverse; PW and PM, proximal wild-type and mutant miR-506 binding site; DW and DM, distal wild-type and mutant miR-506 binding site. Mutated bases are in italics and underlined.