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. 2014 Nov 6;290(1):197–208. doi: 10.1074/jbc.M114.579292

TABLE 2.

Presence of H3 in the Egr1 promoter

The presence of H3 was quantified by RT-PCR at the amplicons indicated, and the results were averaged for each nucleosome.

Amplicon Nucleosome Presence of H3 relative to input
0 min 15 min 30 min
−550 N − 2 6.8 ± 1.2 6.2 ± 0.7 5.9 ± 0.7
−494
−240 N − 1 6.1 ± 0.7 4.8 ± 0.7 3.4 ± 0.4
−178
+171 N + 1 1.9 ± 0.6 6.0 ± 0.2 4.2 ± 0.2
+241