FIGURE 12.

Contributions of different sites to superoxide and H₂O₂ production by isolated skeletal muscle mitochondria ex vivo in media mimicking rest, mild aerobic exercise, and intense aerobic exercise. Positive values taken from Figs. 8D, 9, and 11 were corrected for the losses of H₂O₂ caused by the activity of mitochondrial matrix peroxidases using Equation 1. Because of their topology, only 50% of the signal assessed for sites III_Qo and G_Q was corrected, slightly diminishing their contributions relative to other sites. Inverted error bars indicate the propagated errors for each site, and conventional error bars indicate the propagated sum of these errors. Values are means ± S.E. (error bars) (n = 3–20).