FIGURE 4.
Endocytic pathways of peptide PepL internalization. A, endocytic markers. HEK-293 cells expressing the RFP-fusion proteins (red) indicated were incubated in medium containing 20 μm PepL-DyLight 488 (green). The internalization process was followed by in vivo confocal imaging at the indicated time points. B, early endosome function inhibition. Cells expressing the constitutively active mutant Rab5Q79L were incubated in culture medium containing 20 μm PepL-DyLight 488 and imaged as in A. Scale bar for A and B, 10 μm.