FIGURE 5.

CHD2 binds dsDNA substrates that are at least 40 bp in length. A, electrophoretic mobility gel shift assays (EMSAs) were performed using WT CHD2 and a series of fluorescently-labeled dsDNA probes. Increasing amounts of CHD2 (0, 10, 20, 50, 100, 200, or 400 nm) were incubated with a dsDNA probe (5 nm) of the indicated length. The samples were then resolved by native PAGE and imaged with a fluorescent laser scanner. B, for comparison, the fraction of DNA bound by CHD2 at 200 nm was calculated and graphed. All values are mean and S.D.; n = 3. C, quantification of the fraction of ATP hydrolyzed by CHD2 (100 nm) in the presence of the same dsDNA probes (15, 20, 30, 40, 50, 60 bp) used in gel shift assays after 30 min as compared with fraction of ATP hydrolyzed in the presence of plasmid DNA (∼3 kb). All values are mean and S.D., n = 3.