FIGURE 8.
RhoA mediates activation of the p38 pathway by BMP2/7 in ameloblasts. A, three-dimensional reconstruction of μCT scans from 2-week-old Tak1K14 and WT mice, showing comparable dental cusp morphology. B, ALC cells were infected with lentiviruses expressing control or murine RhoA shRNA. After puromycin selection, RhoA levels were measured by immunoblotting (IB) analysis. C, RhoA-sufficient or -deficient ALC cells were stimulated with BMP2/7 (100 ng/ml) for the indicated duration and immunoblotted with the indicated antibodies. D, RhoA-sufficient or -deficient ALC cells were stimulated with BMP2/7 (100 ng/ml) for 3 h, and RT-PCR was performed to measure gene expression. E, RhoA-sufficient or -deficient ALC cells were transfected with the p21-luc reporter gene and Renilla, and 24 h later cells were stimulated with BMP2/7 for 48 h. p21 luciferase activity was normalized to Renilla. ** indicates p < 0.01 by a two-tailed Student's t test.