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. 2014 Nov 17;290(1):310–324. doi: 10.1074/jbc.M114.606277

FIGURE 4.

FIGURE 4.

MEF2Cα2 promotes myogenesis in C2C12 cells. A, C2C12 cells were transfected with constructs expressing vector, MEF2Cα1, or MEF2Cα2. Expression of the isoforms was confirmed by qRT-PCR. Error bars show mean ± S.D. ***, p < 0.001. B, protein expression of the epitope-tagged MEF2C isoforms was confirmed by Western blot analysis. C, differentiation-specific gene expression is induced by overexpression of MEF2Cα2. Gene expression was assayed for the indicated genes by qRT-PCR. Error bars show mean ± S.D. ***, p < 0.001. D, overexpression of MEF2Cα2 promotes myotube formation, whereas MEF2Cα1 is inhibitory. The cell lines in A were differentiated for 3 days, immunostained with antibodies against MHC, and counterstained with DAPI. Images were taken at ×200 magnification. Scale bars = 5 μm. The data are quantitated in the bottom panels.