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. 2014 Nov 17;290(1):310–324. doi: 10.1074/jbc.M114.606277

FIGURE 5.

FIGURE 5.

MEF2Cα2 promotes differentiation in RMS cells. A, MEF2Cα2 activates a muscle-specific reporter in RMS cells. RD cells were transfected with the indicated constructs. Values are represented with respect to a luciferase vector with no promoter (pGL3 basic). pGL3 (+), luciferase vector with the constitutive CMV promoter; Lmod2-luc, luciferase vector with a ∼300-bp Leiomodin 2 (Lmod2) promoter. Error bars show mean ± S.D. ***, p < 0.001. B, MEF2Cα2 promotes differentiation-specific gene expression in RD cells. RD cells were transfected with constructs expressing vector, MEF2Cα1, and MEF2Cα2. Gene expression was assayed for the indicated genes by qRT-PCR. Error bars show mean ± S.D. ***, p < 0.001. C, MEF2Cα2 promotes the expression of MHC in RD cells. RD cells expressing vector, MEF2Cα1, and MEF2Cα2 were immunostained for MHC and counterstained with DAPI. Images were taken at ×100 magnification. Scale bars = 10 μm.