FIGURE 6.

MEF2Cα1 recruits HDACs to target promoters. A, MEF2Cα1 interacts with HDAC5 more robustly than MEF2Cα2. HEK293 cells were transfected with expression constructs for MEF2Cα1 and MEF2Cα2, immunoprecipitated (IP) with an antibody against MEF2C, and then the blot was probed with antibodies against HDAC5. EXT, extract; exp., exposure. B, MEF2Cα2 inhibits recruitment of HDAC5 to target promoters. ChIP assays were performed on RD cells transfected with vector control or an expression construct for MEF2Cα2 with antibodies against HDAC5, and immunoprecipitated DNA was probed with primers corresponding to the indicated promoters. Error bars show mean ± S.D. ***, p < 0.001. C, HDAC4 recruitment to target promoters is also inhibited by MEF2Cα2. ChIP assays were performed as in B but with antibodies against HDAC4.