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. 2014 Nov 17;290(1):310–324. doi: 10.1074/jbc.M114.606277

FIGURE 7.

FIGURE 7.

Expression of splicing factors in skeletal muscle and RMS. A, ASF is modestly up-regulated upon differentiation in C2C12 cells. Gene expression was assayed by qRT-PCR, and the days of differentiation (D) are indicated. Error bars show mean ± S.D. UD, undifferentiated. B, ASF is highly expressed in RMS cells. Expression was assayed as in A. Hum., human. C, SRPK3 is robustly up-regulated upon differentiation of C2C12 cells, as assayed as in A. D, SRPK3 is highly down-regulated in RMS cells, as assayed as in A. ***, p < 0.001. E, depletion of SRPK3. C2C12 cells were transfected individually with multiple shRNA constructs (shSRPK3), and stable transformants were selected. The two constructs shown are represented by (1) and (2). Gene expression was assayed after 2 days of differentiation by qRT-PCR. Error bars show mean ± S.D. scr, scrambled. ***, p < 0.001. F, SRPK3 is required for MEF2Cα2 splicing. Gene expression was assayed on the shSRPK3 depletions as in E.