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. 2014 Nov 17;290(1):310–324. doi: 10.1074/jbc.M114.606277

FIGURE 8.

FIGURE 8.

SRPK3 and MEF2Cα2 promote differentiation of RMS cells. A, SRPK3 overexpression in RD cells. RD cells were transfected with a vector control or an expression construct for SRPK3 and assayed for gene expression by qRT-PCR. B, SRPK3 induces the expression of MEF2Cα2. Gene expression was assayed by qRT-PCR. Error bars show mean ± S.D. ***, p < 0.001. C, SRPK3 induces differentiation-specific gene expression in RD cells. Cells as in A were assayed for gene expression by qRT-PCR for the indicated genes. Error bars show mean ± S.D. ***, p < 0.001. D, SRPK3 or MEF2Cα2 induce MHC expression in RMS cells. RD (left panel) and RH30 cells (right panel) were transfected with a vector control or expression constructs for SRPK3 or MEF2Cα2, immunostained for MHC, and counterstained with DAPI. Images were taken at ×100 magnification. Scale bars = 10 μm.