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. 2014 Nov 14;290(1):371–383. doi: 10.1074/jbc.M114.616383

FIGURE 3.

FIGURE 3.

Primer extension products with templates bearing the modified nucleosides. Extensions were performed with one dNTP alone (dATP, dTTP, dCTP, or dGTP) or with one dNTP associated with the next incoming nucleotide in its dideoxy form (dATP + ddGTP, dTTP + ddGTP, dCTP + ddGTP, or dGTP + ddGTP), for 10 min. For each compound, “n + 1” (in blue) corresponds to the percentage of primer extended by one nucleoside triphosphate; “n + 2” (in orange) corresponds to the percentage of primer extended by two nucleotides, the first nucleoside triphosphate facing the modified nucleoside; “n + 3” (in pink) is the percentage of primer extended by three nucleotides, the first one facing the modified nucleoside. Incorporation of ddGTP only at position n + 1 is represented by the blue bar on the right-hand side of each histogram.