TABLE 2.
Melting temperatures of matched and mismatched dX/Compl. dY undecamer duplexes
Melting temperatures were measured by fluorescence. Tm was obtained for the matched and mismatched (dX/Compl. dY) duplexes, where X is one of the six modified nucleoside analogues, and Y one of the four natural nucleoside. In boldface type are Tm values obtained for the matched and mismatched natural (dX/Compl. dY) duplexes. Tm (°C) values are the means of three independent experiments ± S.D.
| Compl. dA | Compl. dT | Compl. dC | Compl. dG | |
|---|---|---|---|---|
| dT | 66.4 ± 1.7 | 56.8 ± 1.3 | 54.2 ± 1.4 | 57.9 ± 1 |
| 5-Carbamoyl-dU (1) | 63.7 ± 1 | 54.5 ± 1 | 52.2 ± 1 | 55.2 ± 1 |
| ncm5-dU (2) | 59.6 ± 1.6 | 52.4 ± 1 | 46.2 ± 1 | 55.7 ± 1.2 |
| hm5-dU (3) | 62.3 ± 0.8 | 55.6 ± 1.2 | 53.7 ± 0.4 | 57.8 ± 0.4 |
| ncm3-dU (4) | 50.2 ± 0.5 | 48.6 ± 1 | 48.2 ± 1 | 49 ± 1 |
| dC | 50.9 ± 1.2 | 52.9 ± 1.1 | 52.5 ± 1.3 | 69.3 ± 1.6 |
| ncm5-dC (5) | 47 ± 1 | 46.6 ± 1.3 | 41 ± 1.3 | 62 ± 1.6 |
| hm5-dC (6) | 53.9 ± 0 | 53.6 ± 0.4 | 50.2 ± 0.4 | 66.3 ± 0.6 |
| dA | 54.4 ± 0.7 | 65.8 ± 0.7 | 53 ± 0.6 | 56.2 ± 0.5 |
| dG | 55.1 ± 1.1 | 58.1 ± 1.1 | 68.7 ± 1.5 | 56.3 ± 0.6 |