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. 2014 Nov 17;290(1):448–456. doi: 10.1074/jbc.M114.590364

FIGURE 1.

FIGURE 1.

A and B, real time PCR (A) and Western blot (B) showed much higher expression of both TrpC5 and ABCB1 at the mRNA and protein levels in HCT-8/5-Fu and LoVo/5-Fu cells than in their parental lines (HCT-8 and LoVo cells). *, p < 0.05 (Student's t test). B, Western blot showed more nuclear-stabilized β-catenin accumulation in HCT-8/5-Fu and LoVo/5-Fu cells than in HCT-8 and LoVo cells. *, p < 0.05, (Student's t test). C, immunofluorescent staining showed higher expression of TrpC5 and ABCB1 proteins and more nuclear-stabilized β-catenin accumulation in HCT-8/5-Fu cells than in HCT-8 cells. TrpC5 and ABCB1 proteins were mostly localized near the cell surface. Scale bars, 20 μm.