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A and B, [Ca2+]i measurements using GECO1.2 showed that the TrpC5 in HCT-8/5-Fu (A) and LoVo/5-Fu (B) cells was functional. Shown are the time courses of [Ca2+]i changes in response to La3+ (100 μm). Application of La3+ elicited a rise in [Ca2+]i in HCT-8/5-Fu (A) and LoVo/5-Fu (B) but not in HCT-8 and LoVo cells. The La3+-elicited [Ca2+]i rise in HCT-8/5-Fu and LoVo/5-Fu cells was inhibited by T5E3, a TrpC5-specific blocking antibody. pre IgG, preimmune IgG.
