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. 2014 Nov 17;290(1):448–456. doi: 10.1074/jbc.M114.590364

FIGURE 4.

FIGURE 4.

A, Western blot showed that nuclear accumulation of β-catenin was down-regulated in HCT-8/5-Fu/RNAi cells compared with scrambled siRNA in HCT-8/5-Fu cells, whereas enforcing TrpC5 expression in HCT-8 (HCT-8/TrpC5) cells increased the nuclear accumulation of β-catenin. *, p < 0.05 (Student's t test). B, Western blot showed more nuclear expression of cyclin D1 protein in HCT-8/5-Fu/DMSO than HCT-8 cells. Administration of XAV939 (an inhibitor of the canonical Wnt/β-catenin signal pathway) decreased the nuclear accumulation of β-catenin in HCT-8/5-Fu cells, as well as ABCB1 expression and the nuclear expression of cyclin D1. On the contrary, exposure to LiCl (an activator of the canonical Wnt/β-catenin signal pathway) resulted in more nuclear accumulation of β-catenin, as well as greater nuclear expression of cyclin D1 and ABCB1 membrane expression in HCT-8 cells. *, p < 0.05 (Student's t test). C, Western blot showed that up-regulation of TrpC5 expression in HCT-8 cells led to translocation of β-catenin to the nucleus and up-regulated expression of cyclin D1 and ABCB1. *, p < 0.05 (Student's t test). Whole, whole cell protein; Nuclear, nuclear protein.