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. 2014 Nov 17;290(1):457–466. doi: 10.1074/jbc.M114.616359

FIGURE 1.

FIGURE 1.

Nutlin-3 induces p53-mediated repression of PHGDH. A, Western blot analysis of the protein levels of PHGDH, p53, Mdm2, p21, and Actin in indicated melanoma cell lines (A375, Colo-829, and WM26–64, p53 wild-type; Colo-800 and Sk-mel28, p53 mutant) without (−) or with (+) Nutlin-3 treatment (10 μm) for 2 days. B, the mRNA expression levels of PHGDH in the indicated melanoma cell lines treated with Nutlin-3 (10 μm) were measured using qRT-PCR. Ctrl, control. C, A375-shGFP and A375-shp53 stable knockdown cell lines were treated with Nutlin-3 (10 μm) for the indicated times, and total protein lysates were subjected to Western blot analysis for the expression of PHGDH, p53, p21, and Actin. D, qRT-PCR analysis of PHGDH mRNA levels in A375-shGFP and A375-shp53 cell lines treated with Nutlin-3 (10 μm). All mRNA expression levels were normalized with GAPDH. Error bars represent mean ± S.D. from three experiments.