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. 2014 Nov 17;290(1):457–466. doi: 10.1074/jbc.M114.616359

FIGURE 2.

FIGURE 2.

DNA damage down-regulates PHGDH protein and mRNA levels in a p53-dependent manner. A, expression of endogenous PHGDH, p53, p21, and Actin in the indicated melanoma cell lines without (−) or with (+) treatment of the DNA-damaging agent doxorubicin (0.2 μg/ml). B, qRT-PCR analysis of PHGDH mRNA levels in the indicated melanoma cell lines treated with Dox (0.2 μg/ml). Ctrl, control. C, A375-shGFP and A375-shp53 stable knockdown cell lines were treated with doxorubicin (0.2 μg/ml) for the indicated times, and total protein lysates were analyzed by Western blotting for the expression of PHGDH, p53, p21, and Actin. D, qRT-PCR analysis of PHGDH mRNA levels in A375-shGFP and A375-shp53 cell lines treated with doxorubicin (0.2 μg/ml) for the indicated times. All mRNA expression levels were normalized with GAPDH. Error bars represent mean ± S.D. from three experiments.