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. 2014 Nov 12;290(1):478–491. doi: 10.1074/jbc.M114.593632

FIGURE 6.

FIGURE 6.

Effects of the FAT hinge mutation on Ser910 phosphorylation. A, COS7 cells transfected with HA-tagged WT FAK, R-FAK, or T-FAK were serum deprived for 30 min and then treated for another 30 min with 10 nm bombesin before cell lysis. Phosphorylation of Ser910 and levels of transfected FAK expression were analyzed by immunoblotting with phospho-specific Ser(P)910 (pS910) and HA antibodies (upper panels). In parallel, diphospho-44/42 ERK (pERK) and total ERK were monitored with the corresponding antibodies (lower panels). Molecular mass marker positions are indicated in kDa. B, quantification of three experiments for Ser(P)910 as in A, corrected for the amount of FAK (mean ± S.E.). Two-way ANOVA, interaction; F(2,8) = 5.96, p = 0.026, treatment; F(1,4) = 80.9, p = 0.0008, mutation; F(2,8) = 44.7, p < 0.0001. Sidak's multiple comparisons test, asterisks; ± bombesin, ○; comparisons between FAK constructs, 1 symbol, p < 0.05; 2 symbols, p < 0.01; 3 symbols p < 0.001.