FIGURE 9.

O-Fucose and O-glucose modifications of N function redundantly in the activation of N signaling. A–E′, SOPs in wing discs were detected by anti-Sens antibody staining. SOPs in rumi⁴⁴ homozygote wing discs were isolated from larvae raised at 18 °C (A and A′), 25 °C (B and B′), or 30 °C (C and C′). SOPs in wing discs homozygous for O-fut1^{R245A knock-in} and rumi⁴⁴ were isolated from larvae raised at 18 °C (D and D′) or 25 °C (E and E′). Arrowheads indicate hyperplastic SOPs. Outlines of wing discs are shown by broken lines. A′–E′ show magnified views of the areas in magenta squares in A–E, respectively. Scale bars, 100 μm.